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The plates were designed such that there was no sex bias, and samples from selected sex- and age-matched UFMs were prepared simultaneously to those from ND individuals, in a blinded fashion, and randomised for the order of analysis
Always use proper sterile technique

Sulkanen, S

We found that the addition of DMSO at a final concentration of 12% (v/v) suppressed cell cycle progression in all tested cell lines, while ZIL had little or no effect (Fig