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Washed cells were resuspended and used to inoculate 250 mL of M9 medium supplemented with trace elements

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It was shown that CuET binds to the NPL4, resulting in aggregation of NPL4 and deactivation of P97 segregase which leads to accumulation of misfolded proteins in the cells and ultimately cell death ( The main target of CuET in p97 segregase pathway is NPL4, a key component of p97 segregase
