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Description
The flasks were incubated in a Climo-shaking incubator (Kuhner Ag, Basel, Switzerland) at 110 rpm in a humidified 5% CO 2 /air mixture at 37 C
Homologous recombination into the eosinophil peroxidase locus generates a strain of mice expressing Cre recombinase exclusively in eosinophils
10.1111/ics.12612 108
After washing with PBS, the sections were further incubated with fluorophore-conjugated secondary antibodies at 1:400 dilution for 2 h and then stained with DAPI (10 g/ml) for 10 min at room temperature
