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Western analysis Whole cell extracts from primary myoblasts or C2C12 cells were resolved on a 15% SDS-PAGE gel, transferred to Polyvinylidene difluoride (PVDF) membrane (Bio-Rad), and probed with the following primary antibodies: C/EBP (E299, ab32358, Abcam), MYL (F5, sc-365243, Santa Cruz), MYL12A/B (A-10, sc-376606, Santa Cruz), MYL4 (ab231800, Abcam) and Cyclophilin B (ab16045, Abcam)

Common areas treated with liposuction after a C-section include: Lower abdomen above and around the C-section scar Flanks or love handles. Upper abdomen Back and waistline The procedure can be customized based on your body shape and desired results

For microscopic evaluation liver was fixed in 10% neutral phosphate buffer formalin solution for 48 h