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Description
Briefly, yeast cells were spheroplasted with 500 g ml 1 Zymolyase 100T, homogenized in polyvinylpyrrolidone-buffer (8% polyvinylpyrrolidone, 20 mM potassium phosphate buffer, pH 6.5, 0.75 mM MgCl 2 , and protease and phosphatase inhibitor cocktails) with a Dounce homogenizer
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The canine intervertebral disk: part one: structure and function
Nevertheless, all analytes, including Gln and PGlu, exhibited excellent recovery within the first 24 h after the sample preparation
