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The sequence of primer pairs used for RT-PCR were listed as below: mCol1A1 forward 5-AGCCGCAAAGAGTCTACATG-3, reverse 5-CTTAGGCCATTGTGTATGCAG-3
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Understanding the week-by-week dosage increments Here is a typical week-by-week dosage increment schedule and expected outcomes: Weeks 1-4: The adjustment period The typical starting dose of semaglutide is 0.25 mg once weekly

We measured MMA concentration by LC-MS/MS in whole brain extracts (Group 2 in Fig S1C, Table S1)