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To generate the fibrosarcoma model, animals were orthotopically implanted into the gastrocnemius muscle by intramuscular injection using a 26G needle and a 1 cc syringe with 1 10 7 HT1080 cells suspended in medium without serum or antibiotics

For the FOXO4-DRI treatment group, 50 M FOXO4-DRI (MCE, HY-P4157) was added to the glucose-free DMEM (Gibco, Catalog #11966-025) medium, followed by 3 h of oxygen-glucose deprivation (OGD) treatment

infects the epithelial wall of the intestine and escapes from the innate immunity and ROS activity of the host

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