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Description
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Smith M, Hopkinson DA, Harris H
Spheroplasts were then washed three times with YPD/1 M sorbitol and once with 1 M sorbitol, and lysed as described previously (Waters and Blobel, 1986) with a douncer in lysis buffer [20 mM HEPES pH 7.5, 100 mM KOAc, 2 mM Mg(OAc) 2 , 10% Glycerol, 1 mM DTT, 0.5 mM PMSF, and complete EDTA-free protease inhibitors (Cytiva)]
Verhagen H, van Gils N, Martianez T, van Rhenen A, Rutten A, Denkers F, de Leeuw DC, Smit MA, Tsui ML, de Vos Klootwijk LLE, et al
