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An entropic safety catch controls hepatitis C virus entry and antibody resistance

Step 8 Label and Store the Reconstituted Peptide Use a permanent marker to write on the vial: Date of reconstitution Compound name Concentration (mg/mL) Volume added Store the labeled vial in a refrigerator set to 3646F (28C)

A., Brenner, C

To assess whether RT probe binds to any proteins or reacts with free cysteine residues in some proteins that may interfere with the final ratio readouts, we incubated RT with cell lysate, which was dialyzed using a 3 K cut-off membrane to remove all small molecules including GSH, and observed no significant changes in fluorescent signal ratios even at the highest lysate concentration shown (protein concentration is 36 mg ml 1 based on a BCA assay), suggesting that there is no non-specific interactions or reactions between cellular proteins and RT which could significantly change the ratio readouts (Fig