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Bone-marrow-derived mature neutrophils were isolated by positive selection using an anti-Ly6G MicroBead kit or anti-Ly6G MicroBeads UltraPure purification kit (Miltenyi Biotec, 130-120-337)
The serum cobalamin concentration does not accurately reflect the real availability of cobalamin, because cobalamin-dependent metabolic reactions occur within cells, mainly in the cytoplasm and mitochondria [15]
Acta 1313, 130138 10.1016/0167-4889(96)00063-8 [DOI] [PubMed] [Google Scholar] 47.Miyamura Y., Coelho S.G., Wolber R., Miller S.A., Wakamatsu K., Zmudzka B.Z.et al
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