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AMPK) 14,15

There is a long extracellular loop consisting of 107 amino acids between the first two transmembrane domains, which is highly conserved among organic cation transporters encoded by members of the solute carrier (SLC) 22 family, suggesting an essential role in transporter function [92]

in acute and chronic prostatitis and also as an adjuvant to antibiotic therapies recommended in cases of bacterial prostatitis

Cells were lysed with NIB-250 buffer (15 mM Tris-HCl (pH 7.5), 60 mM KCl, 15 mM NaCl, 5 mM MgCl 2 , 1 mM CaCl 2 , 250 mM sucrose, 1 mM DTT, 10 mM sodium butyrate, protease inhibitors) with 0.1% NP-40 for 5 minutes on ice