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ERK1/2 phosphorylation assays and NF-B phosphorylation assays Changes in phosphorylated ERK1/2 (p-ERK) were detected using the AlphaScreen SureFire p-ERK1/2 Kit following the methods described in detail elsewhere [31, 32]

The reduction in IL-6 may be due to the Mediterranean diets antioxidant potential and ability to raise BMI [124]
Proteins, small peptides and other signaling molecules identified as inconspicuous but possibly important players in microspores reprogramming toward embryogenesis
