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Based on previously published methods (Chen and Asada, 2009, 2013), SOD (EC 1.15.1.1), CAT (EC 1.11.1.6), and APX (EC 1.11.1.11) activities were assayed

To elucidate the function of the [4Fe4S] cluster in catalysis, the four cysteine residues (C300, C304, C308, C320) that structure the [4Fe4S] cluster were mutated to serine or alanine

This could represent a useful marker to test the efficacy of agents which may increase BBB integrity

doi: 10.7759/cureus.31851 164 AbhishekBRVelayuthamSSJeyarajKMSowminiPRKumarMSSarvananSVet al
