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Brain 142:391411 Sun HL, Deng DP, Pan XH, Wang CY, Zhang XL, Chen XM, Wang CH, Liu YX, Li SC, Bai XY, Zhu W (2016) A sub-threshold dose of pilocarpine increases glutamine synthetase in reactive astrocytes and enhances the progression of amygdaloid-kindling epilepsy in rats

This is due to several factors that differ between the controlled conditions of in vitro studies and the complex biological processes in living organisms, including interactions between different cell types, organ systems, and environmental variables (e.g., gut microbiota or circulation) that can influence how a drug works

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