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The slices were rinsed and placed into a chamber embedded in the stage of an Olympus BX51WI microscope (Olympus) coupled to a 12-bit CCD fluorescent camera (SensiCam, PCO imaging)

In the body, thioctic acid activates a protein that binds to glutathione and works as an antioxidant

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The presence of a vaginal plug was considered as evidence of fertilization, and the day the plug was observed was regarded as embryonic day (E) 0, the first embryonic day
