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Clin Endocrinol 2011;1:13-23

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After the cross-linking reaction, 10 L of the reaction mixture was suspended into an equal volume of sample buffer for SDS-PAGE (Nacalai-Tesque) analysis, incubated for 3 min at 100 C, loaded onto a 12% SDS-PAGE gel, and the gel was stained by CBB stain One Super (Nacalai-Tesque)

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A review of nitric oxide and oxidative stress in typical ovulatory women and in the pathogenesis of ovulatory dysfunction in PCOS

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It just requires a consistent, disciplined process

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