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Then AGO1 antibody, AGO2 antibody, and control IgG were added to the cytoplasmic fraction and the nuclear fraction, respectively
Afterwards, the synaptosomes were washed with PBS, fixed with 0.25% paraformaldehyde (PFA), and stored in the dark at 4 C
Supplementary Figs S5 and S6 and Supplementary methods)
Shamana therapy involves the administration of herbal formulations including Adhatoda vasica (Vasaka), Piper longum (Pippali), and Glycyrrhiza glabra (Yashtimadhu), which are traditionally used to alleviate respiratory symptoms and enhance pulmonary function
