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The triacylglycerol hydrolysis reaction catalyzed by lipases generally can be written as: The activity of lipases can be examined by the monitoring of release of either free fatty acids or glycerol from triacylglycerols or fatty acid ester displays by this reaction [148]

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It is therefore not surprising that already the addition of a residue at the N-terminus of W1 turns off the enzymatic activity of Lm PC-PLC 51 , since the placement of a new residue would require a change in the position of W1