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doi: 10.1002/ptr.5188

Native NMT1-ribosome complex isolation A plasmid for transient expression of NMT1 was generated by inserting the coding sequence of NMT1 which was amplified from cDNA into a pcDNA5/FRT/TO mammalian expression vector (Thermo Fisher Scientific) that was modified to contain a N-terminal 3xFLAG-3C cleavage site tag via restriction cloning

PDK family proteins predominantly locate within the mitochondrial matrix and have four members with distinct tissue or cell type specificity

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