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However, an increase in mutation frequency above a critical threshold (usually 60%80%) leads to pathogenicity and mitochondrial disease manifestations ( 3.2 Oxidative stress Although many chronic diseases are multifactorial in origin, they all are associated with a common process, namely oxidative stress

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Subsequently, NK or CD8 + T cells (effector cells) were added and incubated for 6 h at an E: T ratio of 3:1 45 , followed by incubation with propidium iodide solution for 5 min at RT