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Some of these factors include your skin type, tone, layering of the tattoo, location, pigmentation, the amount of ink applied to your tattoo, the kind of pigment used, whether homemade pigment or professional ink was used, and any pre-existing scars from the tattoo
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The lysates were centrifuged at 4 C for 5 min, and supernatants were incubated with anti-SIRT1 (Ab110304, Abcam) or anti-SIRT3 (Ab246522, Abcam) antibodies for 4 h at 4 C
