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This procedure is preferred to the use of washed acid precipitated proteins as we noted that, after restoring neutral pH, the presence of a massive amount of denatured hemoglobin induces oxidation of the reducing agent used to detach GSH from GSSP, hampering a precise and accurate measurement of GSSP

doi:10.1084/jem.140.5.1147

Claudin proteins are small (2027 kDa) transmembrane proteins expressed in tissue- and cell-specific manner, 12, 13 with a total of 27 claudin family members having been identified to date

Four hours after N-acetylcysteine administration, intracellular glutathione concentrations in the patients were moderately higher than at baseline and at 2 h