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The resulting mixture was electroporated (pulse voltage, 1,600 V

Furthermore, AMPK-dependent phosphorylation of NAMPT enhances its activity to generate NAD + from phosphoribosyl pyrophosphate (PRPP) (Liao et al., 2022)

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The authors sincerely thank Professor Graham Rook (University College London, United Kingdom) for discussions on his Old Friends Hypothesis and critical reading of the manuscript, and Professor Hanna Kmita (Adam Mickiewicz University, Poznan, Poland) for a critical reading of the manuscript and helpful suggestions for its improvement