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This protocol has been applied to several cell lines challenged with a pro-ferroptotic agent and several PE-lipoxidated proteins have been detected and identified, including those present in the plasma membrane

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doi:10.1007/s40618-017-0755-2 Collins AB, Pawlak R

Adult mouse astrocyte isolation Brain tissues were aseptically removed, minced and enzymatically digested in HBSS (Gibco, 14175103) containing 50 mg ml 1 collagenase D (Sigma-Aldrich, 11088858001), 100 g ml 1 TLCK trypsin inhibitor (Sigma-Aldrich, T9378), 100 U l 1 DNase I (Sigma-Aldrich, D5025), and HEPES pH 7.2 (Gibco, 15630080) for 1 h at 23 C with shaking