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Moreover, the study reinforced the importance of P53 in eliminating telomere-damaged cells and its impact on aging [206]

The cells were first stained using Fixable Viability Stain 510 (FVS, 1:1000), which was followed by surface staining with anti-APC-CyTM7 CD45 BV510 antibodies (1:1000), anti-FITC-mouse CD3 antibodies (1:50), anti-PerCP-Cy5.5-mouse CD4 antibodies (1:50), and intracellular cytokines were then stained with anti-PE-mouse IFN antibodies (1:50) and anti-APC-mouse IL-4 (1:50) or anti-PE-mouse IL-17A antibodies (1:50)

[PubMed] [Google Scholar] 17.Simon JA, Hudes ES

The study protocol was approved by the ethics committee of Louisiana State Health Sciences Center-Animal Care Committee (Animal Care protocol number P-15-006), and all the methods were carried out following the approved guidelines