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Immunoblot assay Following extraction of genomic DNA, the samples were denatured at 100 C for 10 min, and 500 ng per well was spotted onto nitrocellulose membranes (Bio-Rad, Hercules, CA, USA) using a slot-blot apparatus (Omniphor, San Jose, CA, USA)

[2] These have less study done into their effects as of yet, but initial results seem promising

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Investigations have revealed that, under hypoxic conditions, in antigen-presenting cells lacking HIF-1, overexpression of STAT3 significantly inhibits Th1 proliferation (93)
