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Thin sections were cut using an ultramicrotome (Leica Microsystems, Germany), and the sections were subjected to double staining with uranyl acetate and lead citrate and then observed using a transmission electron microscope (Hitachi HT7800, Japan)
The CD spectra were measured at an enzyme concentration of 2 M with a 1 mm path length cell at 25C
doi: 10.3389/fnut.2020.00101 33
References 1 MantovaniAAllavenaPMarchesiFGarlandaC
