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(14)C-DTFA, 10 to 15mg with a (14)C activity of 15 to 20 pCi, was administered IV to 4 patients

Methods Cell culture and transfection Human cervical adenocarcinoma cells (HeLaM cells were a gift from Roger Tsien, University of California, San Diego) were cultured in RPMI-1640, retinal pigment epithelial (hTERT-RPE1 purchased from ATCC, CRL-4000) cells in 1:1 mixture of DMEM and F12 HAM Media and A431 cells (purchased from the European Collection of Authenticated Cell cultures, 85090402) in DMEM

Research has shown that antioxidant interventions, including vitamin E, deferoxamine (DFO), or Fer-1, can alleviate ferroptosis in T cells lacking GPX4 ( Figure 2 ) (114)