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cDNAs were reverse transcribed in the presence of a mixture of oligo dT and random primers (9:1) at 50C for 50 min using ThermoScript Reverse Transcriptase or SuperScript IV Reverse Transcriptase (Invitrogen) following the manufacturers instructions

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Endocrine lineage biases arise in temporally distinct endocrine progenitors during pancreatic morphogenesis

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Effective system planning must also include robust cross-border arrangements for shared catchments such as the Severn and the Dee

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