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In aged mice (2531 months), MTN numbers are significantly reduced, with marked nuclear enlargement and lipofuscin accumulation, whereas adjacent masseter motoneurons display loss of Nissl bodies (Sturrock, 1987)
However, results presented here are not mutually exclusive with the possibility of bacterial metabolism playing an important role in metformins effect on aging, when appropriate gelling agents are used
Remove needle, discard safely, wipe area with alcohol Step 7
doi: 10.4049/jimmunol.0804003 133 HsiehWYZhouQDYorkAGWilliamsKJScumpiaPOKronenbergerEBet al
