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BT474 cells and PC3- hTERT cells were cultured in DMEM F-12 medium (Gibco, Invitrogen) with 10% FBS (Gibco, Invitrogen) and 1% PenicillinStreptomycin (Gibco)

In an attempt to obtain more robust prognostic information, Ki-67/KIT double-staining was previously evaluated, and was shown to be valuable for prognosis [142]
