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After wash Triton several times, the coverslips were incubated with the conjugated antibody Phalloidin-iFluor 488 (Abcam, ab176753) in a wet chamber for 1 hour at RT

Garlics potential effect on HCy levels has been reported in a small clinical trial of atherosclerosis patients randomized to aged garlic extract ( P =0.08).127 Additionally, in an animal model of HHCy, induced by a severely folate-depleted diet in rats, aged garlic extract decreased plasma HCy concentrations by 30%.128 In contrast, elevated levels of HCy caused by mild folate deficiency did not change significantly by garlic supplementation.128 Thus, garlic may have an effect on HCy metabolism independent of the effect of B vitamins in addition to boosting H 2 S production

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However, the line between a k-hole experience and an overdose is a thin one