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BurgessECrakeRPhillipsEMorrinHRoydsJSlatterTet alIncreased ascorbate content of glioblastoma is associated with a suppressed hypoxic response and improved patient survival

Loredana Ungureanu E, Mustatea G

(2020) 53:e12891

For protein purification, harvested cells were resuspended with ice-cold lysis buffer (50 mM Tris-Cl pH 8.0, 300 mM NaCl, and cOmplete, EDTA-free protease inhibitor cocktail tablets (Roche)) at a ratio of 3.2 mL/g of cell pellet
