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After all nonretained components are washed off the column, the retained solute or together with ligand as solute-ligand complex can be eluted by applying a solvent

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After administering the Sirt1 inhibitor EX527 to CSDS mice, levels of Nrf2, HO-1, and GPX4 decrease, suggesting that Sirt1 may enhance antidepressant effects via the Nrf2/HO-1/GPX4 pathway