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Description
All GSLs feature an O -sulfated ( Z )-thiohydroximate core connected to a -D-glucopyranose unit and an amino acid-derived side-chain, with their structural diversity stemming from their variable side-chain origin and modification during biosynthesis
Generation of HeLa cells stably expressing APEX2 For generation of stable cell lines, the pLPCX retroviral expression vector encoding APEX2-GFP or mito-APEX2 (Su9-APEX2) was transfected into the packaging cell line Phoenix-AMPHO
(Huang et al., 2015
Unfortunately, none of the GSH1ox lines expressed the GSH1 protein and were omitted from further analysis
