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Cell-mediated immunity plays a central role, with nave CD4 + T cells differentiating into effector subsets upon antigen recognition: Th1 cells secrete IFN-, IL-2, and TNF-, inducing MHC class II expression and promoting Fas-mediated apoptosis of thyrocytes

In both cases, cells were then stained with propidium iodide (PI) at room temperature for 1 h, in PBS containing 20 g/mL PI (Sigma-Aldrich), 200 g/mL RNase A and 0.1% Triton X-100

The maintenance of the two distinct subpopulations is an effective strategy to face different environmental conditions found in the life styles of B

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