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The crude enzyme extract (0.1 mL) was pooled with phosphate buffer at a concentration of 50 mmol L 1 (pH 5.0, 500 L), deionised water (0.8 mL), riboflavin at a concentration of 0.6 mol L 1 (0.1 mL), nitroblue tetrazolium sodium at a concentration of 20 mol L 1 (0.1 mL), 22 mol L 1 methionine (0.2 mL) and Triton-X at a concentration of 0.1 mol L 1 (0.2 mL)

CRISPRCas9 MTA1 inactivation by individual sgRNAs Inactivation of MTA1 was carried out by cloning sgRNAs into plentiCRISPRv2 (Addgene, 52961) plasmid

A formulation that combines amino acid coating powder and glutathione essence

[DOI] [PubMed] [Google Scholar] 2.Jones D.P