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For chromatographic metabolite separation, a Vanquish ultra-performance liquid chromatography (UPLC) system was coupled to a Q Exactive HF (QE-HF) mass spectrometer equipped with heated electrospray ionization (HESI

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Although these studies can all reflect the magnitude of radiation received by the human body under specific conditions, when using RNA as a radiation biodosimeter in practical applications, it is necessary to consider the situation caused by individual differences, and to exclude nonspecific transcriptomic changes brought about by factors such as gender [176], age [177], and environment [178]
