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All assays were previously checked for specificity (melting curve and agarose gel electrophoresis of PCR products) and reaction efficiency, dynamic range and linearity (standard curve experiments) and reproducibility (estimation of the coefficient of variation-CV by analysing a series of samples in different runs)

Although a dose-related increase in urinary NAcLys-[ 13 C 4 ]-BDA excretion was observed, correlation analysis was not performed for NAcLys-[ 13 C 4 ]-BDA, as accurate analysis of this metabolite was only possible in the two highest dose groups due to an interfering peak

Clinical behaviors frequently associated with copper overload autism Sudden emotional outbursts without clear external trigger High irritability or agitation during noise, transitions, or crowds Panic or fear-based behavior Sensory oversensitivity (especially auditory) Hyperactivity alternating with emotional collapse Cyclical mood swings Poor tolerance to stimulants or SSRIs These patterns represent a neurochemical imbalance not behavioral stubbornness or lack of discipline

From a public health perspective, the pervasive presence of pesticide residues in food and the environment highlights the urgent need for stricter regulatory enforcement, continuous biomonitoring, and the adoption of safer agricultural practices