intracellular glutathione metabolism NRF2 and cancer Glutathione metabolism in cancer progression

Serial dilution spot assays and growth curves In a typical growth experiment, single colonies of the WT, gsh2 mutants, or gsh2::GSH2 strains were selected from an agar plate and incubated at 30C in YPD medium overnight with shaking at 220 rpm

The relative intensity of Western blot bands was quantified by density analysis using ImageJ software and normalized against an internal control protein to assess the expression level of the target protein

Figure 1: Photographs of Emil von Behring (left) and Shibasabur Kitasato (right) together with a vial of serum therapy against diphtheria dating back to 1895

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