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METHOD DETAILS Plasmids For bacterial expression, human TOM1 (Horizon MGC cDNA MHS6278-202760163), human TOM1L1 (Horizon MGC cDNA MHS6278-202840799) and human TOM1L2 (pGFP-TOM1L2, short isoform, gift from Folma Buss (Tumbarello et al., 2012)) were amplified and cloned into pGPS1 (GST-PreX-Stag-) by conventional cloning

Research subjects should be fully informed about: The nature and purpose of the research Possible risks and adverse effects Tracking procedures Right to withdraw from research Confidentiality protections Contact data for questions or concerns Regulatory Considerations Researchers should be aware of control frameworks governing peptide research in their jurisdiction