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For flow cytometry analysis, cells were stained in a surface antibody mix in PBS for 25 minutes at 4C, washed in PBS, then fixed/ permeabilized for 1hr at 4C using eBioscience Foxp3/ Transcription Factor Staining Buffer Set (Thermo Fisher Scientific)
There is currently no universally accepted therapeutic dosage for BPC 157
However, they are known to have a number of side effects and may adversely affect the health of a patient
