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To determine whether elevated CCK expression within the mPFC underlies the increased vulnerability to SDS-induced anxiety in adult KI mice, we used a viral-mediated short hairpin RNA (shRNA) strategy driven by the CaMKII promoter to selectively attenuate Cck expression in mPFC excitatory neurons

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To investigate this possibility, we used high-resolution respirometry and analysed various respiratory states in the striatal tissues of mutants and controls at 3 months and 8 months

Briefly, estradiol plus BSO-treated MCF-7:2A cells were washed twice with PBS and incubated with 1 g/mL rhodamine 123 at 37C for 30 min