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Plasmid DNA (1 g) expressing the gss gene was transfected into DF-1 cells mediated by Lipofectamine 3000 (Invitrogen) as the manual

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This results from a tyramine-induced depletion of norepinephrine normally present in the readily releasable vesicle pool, and replacement of vesicular norepinephrine with tyramine (which functions as a false- or pseudo- neurotransmitter), which when released has no effect on postsynaptic adrenergic receptors (Figure 1)