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Issues like cost, availability, side effects (such as nausea), and the simple fact that obesity is a complex condition drive the search for other solutions
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Briefly, cells are cultured with the STEMdiff TM definitive endoderm basal medium with supplements A and B for 24h followed by STEMdiff TM definitive endoderm basal medium with supplement B for 48h with daily medium change (Xia et al., 2017)
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