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doi: 10.1038/s41582-021-00530-8 62 HeissE.HerhausC.KlimoK.BartschH.GerhuserC
Superoxide reporters of the DHE family yield non-specific raw fluorescence, thus quantification must rely on chromatographic separation of DHE2-hydroxyethidium (2-OH-E) by HPLC to measure O 2 and discriminate it from ethidium or other adducts, ideally alongside SOD controls and pathway-selective perturbations (e.g., NOX2 inhibitors, mitochondrial site inhibitors) for source assignment
