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Western immunoblot analysis The serum samples and liver homogenates (previously prepared for determination of oxidative stress biomarkers) were separated by 12% SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Hybond-P, Amersham Pharmacia Biotech), blocked in a 0.2% Tween 20, 50 mM Tris HCl pH 7.6, and 150 mM NaCl solution containing 5% non-fat condensed milk

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