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After three basal measurements, cells were permeabilized by injection of saponin (1 g/mL, Sigma S4521), together with 1 mM ADP (Sigma 01905) and the following respiratory complex substrates: CI, pyruvate/malate (5 mM/2.5 mM)
Zhang X, Yang F, Han L, Su Q, Gao Y, Wu R, et al
A combination of such factors could likely cause many cells to destroy themselves
