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Proline is thought to protect membranes and proteins from high concentrations of inorganic ions and harsh temperatures, as well as to stabilize cellular structures, detoxify free radicals and store carbon (C), nitrogen (N2), and energy (Hare and Cress, 1997)

The assay is composed of two parts: the preparation of cell cytosolic/tissue extracts and the detection of total glutathione (GSH and GSSG)

However, these therapies also have many side effects
