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Clinically supervised topical use, with pharmaceutical-grade formulations, is the safest approach

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S-nitrosylation of peroxiredoxin II E promotes peroxynitrite-mediated tyrosine nitration

Then, 20 L of MTT solutions was added to each well followed by an incubation period of 3 hours, then the media were removed and 200 l of DMSO was added to dissolve the crystal and the absorbance was recorded at 570 nm (BioTek, USA) and cell viability was calculated as a percentage compared to cells treated with DMEM alone
